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ah3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ah3
    Ah3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ah3/Histone+H3+Antibody/10__1158_slash_0008___5472__can___22___0736-55-21-22
    Average 97 stars, based on 4296 article reviews
    ah3 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Trichostatin A inhibits inflammation in phorbol myristate acetate‑induced macrophages by regulating the acetylation of histone and/or non‑histone proteins.
    Article Snippet: Briefly, 50 μg protein extracts were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). .. The membranes were blocked with 5% skim milk for 1 h and then incubated with the following polyclonal primary antibodies at 4 ̊C overnight: Anti-HDAC1 (cat. no. 2062; 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-HDAC2 (cat. no. 2540; 1:1,000; Cell Signaling Technology, Inc.), anti-HADC3 (cat. no. 2632; 1:1,000; Cell Signaling Technology, Inc.), anti-H2A (cat. no. 2578; 1:1,000; Cell Signaling Technology, Inc.), anti-AH2A (cat. no. 2576; 1:1,000; Cell Signaling Technology, Inc.), anti-H2B (cat. no. 2934; 1:1,000; Cell Signaling Technology, Inc.), anti-AH2B (cat. no. 2571; 1:1,000; Cell Signaling Technology, Inc.), anti-H3 (cat. no. sc-8654; 1:500; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-AH3 (cat. no. 8172; 1:1,000; Cell Signaling Technology, Inc.), anti-H4 (cat. no. 2935; 1:1,000; Cell Signaling Technology, Inc.), anti-AH4 (cat. no. 2591; 1:1,000; Cell Signaling Technology, Inc.), anti-p65 (cat. no. 6956; 1:1,000; Cell Signaling Technology, Inc.), anti-phosphorylated-p65 (cat. no. 3031; 1:1,000; Cell Signaling Technology, Inc.), anti-Ac-p65 (cat. no. 3045; 1:1,000; Cell signaling Technology, Inc.), anti-TLR4 (cat. no. sc-10741; 1:500; Santa Cruz Biotechnology, Inc.) and anti-β-actin (cat. no. 4967; 1:1,000; Cell Signaling Technology, Inc.). .. This was followed by incubation with a fluorescent secondary antibody (LI-COR Biosciences, Lincoln, NE, USA) at 37 ̊C for 2 h. The blot was analyzed using the Odyssey Infrared Imaging system (LI-COR Biosciences).



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    Antibodies used in this study.

    Journal: International Journal of Molecular Sciences

    Article Title: BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis

    doi: 10.3390/ijms23041985

    Figure Lengend Snippet: Antibodies used in this study.

    Article Snippet: Ac-Histone H3 (AH3-120) Mouse mAb , Santa Cruz , sc-56616, WB: 1:500.

    Techniques: Generated

    CAY10683 alleviates liver damage through regulating the mitochondrial apoptotic pathway in ALF rats. (a) The RFU value was detected by the purified MPTP fluorescence detection kit. (b) The mRNA level of bcl2 and bax mRNA levels were detected by RT-PCR. (c-e) The protein expressions of HDAC2, cyt c in mitochondrion, cyt c in cytosol, caspase 3, c-caspase 3, caspase 9, c-caspase 9, apaf1, bcl2, and bax were detected by western blot. (f) The protein expressions of AH3 and H3 were detected by western blot. # P < 0.05, compared with the control group. ∗ P < 0.05, compared with the LPS/D-gal-treated model group.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Modulations of Histone Deacetylase 2 Offer a Protective Effect through the Mitochondrial Apoptosis Pathway in Acute Liver Failure

    doi: 10.1155/2019/8173016

    Figure Lengend Snippet: CAY10683 alleviates liver damage through regulating the mitochondrial apoptotic pathway in ALF rats. (a) The RFU value was detected by the purified MPTP fluorescence detection kit. (b) The mRNA level of bcl2 and bax mRNA levels were detected by RT-PCR. (c-e) The protein expressions of HDAC2, cyt c in mitochondrion, cyt c in cytosol, caspase 3, c-caspase 3, caspase 9, c-caspase 9, apaf1, bcl2, and bax were detected by western blot. (f) The protein expressions of AH3 and H3 were detected by western blot. # P < 0.05, compared with the control group. ∗ P < 0.05, compared with the LPS/D-gal-treated model group.

    Article Snippet: Rabbit anti-rat/human HDAC2, bcl2, cytochrome c (cyt c), apoptosis protease activating factor 1 (apaf1), caspase 3/cleaved-caspase 3, caspase 9, cleaved-caspase 9, histone H3 (H3), and acetylated histone H3 (AH3) antibodies were obtained from Cell Signaling Technology (Boston, USA).

    Techniques: Purification, Fluorescence, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control